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Impact of CNF/ZnO nanohybrids on bacterial viability assessed using Live/Dead staining assays. S. aureus cells were exposed to CNF/ZnO nanohybrids (50–400 µg/mL) at 37 °C for 2 h ( A ) and 5 h ( B ). Data are expressed as the percentage of dead cells, calculated from the ratio of EthD-III fluorescence intensity (dead cells) to DMAO fluorescence intensity (total cells) (mean ± SD, n = 3). Statistical significance of differences between a-CNF/ZnO and c-CNF/ZnO hybrids (Student’s paired two-tailed t -test) is indicated as * p < 0.05, *** p < 0.001, and **** p < 0.0001; absence of annotation indicates no significance ( p > 0.05).

Journal: Materials

Article Title: Bio-Derived Cellulose Nanofibers for the Development Under Environmentally Assessed Conditions of Cellulose/ZnO Nanohybrids with Enhanced Biocompatibility and Antimicrobial Properties

doi: 10.3390/ma19020346

Figure Lengend Snippet: Impact of CNF/ZnO nanohybrids on bacterial viability assessed using Live/Dead staining assays. S. aureus cells were exposed to CNF/ZnO nanohybrids (50–400 µg/mL) at 37 °C for 2 h ( A ) and 5 h ( B ). Data are expressed as the percentage of dead cells, calculated from the ratio of EthD-III fluorescence intensity (dead cells) to DMAO fluorescence intensity (total cells) (mean ± SD, n = 3). Statistical significance of differences between a-CNF/ZnO and c-CNF/ZnO hybrids (Student’s paired two-tailed t -test) is indicated as * p < 0.05, *** p < 0.001, and **** p < 0.0001; absence of annotation indicates no significance ( p > 0.05).

Article Snippet: Viability/Cytotoxicity Assay Kit for Bacteria Live & Dead Cells was purchased from Biotium (Hayward, CA, USA).

Techniques: Staining, Fluorescence, Two Tailed Test